Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules

Authors
Kasai, K Fujie, M Nakanishi, Y Murooka, Y Usami, S Yamada, T
Citation
K. Kasai et al., Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules, J BIOSCI BI, 89(6), 2000, pp. 559-563
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
89
Issue
6
Year of publication
2000
Pages
559 - 563
Database
ISI
SICI code
1389-1723(200006)89:6<559:MCACOT>2.0.ZU;2-V
Abstract
Two cDNAs that encode asparagine synthetase were cloned from root nodules o f Astragalus sinicus cv. Japan (Renge-sou). The expression of the transcrip ts was nodule-enhanced. The expression of both genes was reduced in nodules when (NH4)(2)SO4 was added to the culture medium. This is the first report of the inhibition of asparagine synthetase gene expression by ammonium in root nodules.