EFFECT OF ALPHA-TOCOPHEROL OVER A WIDE CONCENTRATION RANGE (10(-17)-10(-2)M) ON PROTEIN-KINASE-C ACTIVITY - RELATIONSHIP TO CELL-PROLIFERATION AND TUMOR-GROWTH

Authors
PALMINA NP MALTSEVA EL KURNAKOVA NV BURLAKOVA EB
Citation
Np. Palmina et al., EFFECT OF ALPHA-TOCOPHEROL OVER A WIDE CONCENTRATION RANGE (10(-17)-10(-2)M) ON PROTEIN-KINASE-C ACTIVITY - RELATIONSHIP TO CELL-PROLIFERATION AND TUMOR-GROWTH, Biochemistry, 59(2), 1994, pp. 135-139
Citations number
34
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
2
Year of publication
1994
Pages
135 - 139
Database
ISI
SICI code
0006-2979(1994)59:2<135:EOAOAW>2.0.ZU;2-8
Abstract
The effect of the natural antioxidant alpha-tocopherol (alpha-TP) on t he activity of protein kinase C (PK-C) isolated from rabbit heart has been studied. alpha-Tocopherol inhibits PK-C activity; the concentrati on dependence curve has two maxima, at 10(-6)-10(-4) M and at 10(-15)- 10(-11) M, with a ''silent zone'' between them. Protein kinase C activ ated by preincubation with 12-O-tetradecanoylphorbol 13-acetate (TPA) shows a shift of first maximum towards higher alpha-TP concentrations (5.10(-3) M), and the value of both inhibition maxima decreases from 8 0-90 to 40-60%. The putative role of alpha-TP and lipid hydroperoxides in the regulation of normal and tumor cell proliferation is discussed .