Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51

Citation
Dr. Ewalt et Bj. Bricker, Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51, J CLIN MICR, 38(8), 2000, pp. 3085-3086
Citations number
5
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
8
Year of publication
2000
Pages
3085 - 3086
Database
ISI
SICI code
0095-1137(200008)38:8<3085:VOTABA>2.0.ZU;2-U
Abstract
The Brucella AMOS PCR assay was previously developed to identify and differ entiate specific Brucella species. In this study, an abbreviated Brucella A MOS PCR test was evaluated to determine its accuracy in differentiating Bru cella abortus into three categories: field strains, vaccine strain 19 (S19) , and vaccine strain RB51/parent strain 2308 (S2308). Two hundred thirty-on e isolates were identified and tested by the conventional biochemical tests and Brucella AMOS PCR. This included 120 isolates identified as B. abortus S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus b iovar 1, 15 identified as B. abortus by. 2, 1 identified as B. abortus by. 2 (M antigen dominant), 7 identified as B. abortus by. 4, and 22 identified as B. abortus S2308 and isolated from experimentally infected cattle. The Brucella AMOS PCR correctly identified each isolate as RB51/S2308, S19, or a field strain of Brucella.