Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51
Dr. Ewalt et Bj. Bricker, Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51, J CLIN MICR, 38(8), 2000, pp. 3085-3086
The Brucella AMOS PCR assay was previously developed to identify and differ
entiate specific Brucella species. In this study, an abbreviated Brucella A
MOS PCR test was evaluated to determine its accuracy in differentiating Bru
cella abortus into three categories: field strains, vaccine strain 19 (S19)
, and vaccine strain RB51/parent strain 2308 (S2308). Two hundred thirty-on
e isolates were identified and tested by the conventional biochemical tests
and Brucella AMOS PCR. This included 120 isolates identified as B. abortus
S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus b
iovar 1, 15 identified as B. abortus by. 2, 1 identified as B. abortus by.
2 (M antigen dominant), 7 identified as B. abortus by. 4, and 22 identified
as B. abortus S2308 and isolated from experimentally infected cattle. The
Brucella AMOS PCR correctly identified each isolate as RB51/S2308, S19, or
a field strain of Brucella.