Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci

Authors
Kariyama, R Mitsuhata, R Chow, JW Clewell, DB Kumon, H
Citation
R. Kariyama et al., Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci, J CLIN MICR, 38(8), 2000, pp. 3092-3095
Citations number
14
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
8
Year of publication
2000
Pages
3092 - 3095
Database
ISI
SICI code
0095-1137(200008)38:8<3092:SARMPA>2.0.ZU;2-R
Abstract
Conditions have been optimized for the use of a multiplex PCR for the detec tion of vancomycin-resistant enterococci in nosocomial surveillance specime ns. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enter ococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA ) were used in one reaction tube. The PCR method developed in the present s tudy is simple and reliable for the rapid characterization of vancomycin-re sistant enterococci.