Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribosomal DNA

Authors
Motoyama, AB Venancio, EJ Brandao, GO Petrofeza-Silva, S Pereira, IS Soares, CMA Felipe, MSS
Citation
Ab. Motoyama et al., Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribosomal DNA, J CLIN MICR, 38(8), 2000, pp. 3106-3109
Citations number
23
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
38
Issue
8
Year of publication
2000
Pages
3106 - 3109
Database
ISI
SICI code
0095-1137(200008)38:8<3106:MIOPBB>2.0.ZU;2-#
Abstract
We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and in tergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using prime rs specifically designed for both ribosomal DNA regions, we were able to di scriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomyco sis diagnosis and ecological and molecular epidemiological studies of P. br asiliensis in Latin America.