A GENERAL-METHOD FOR PURIFICATION OF HUMAN RECOMBINANT SOMATOTROPIN AND ITS MUTANT FORMS BY METAL CHELATE AFFINITY-CHROMATOGRAPHY

Authors
LOPATIN SA ILINA AV SHULGA AA GRINCHENKO OS VARLAMOV VP SKRYABIN KG KIRPICHNIKOV MP
Citation
Sa. Lopatin et al., A GENERAL-METHOD FOR PURIFICATION OF HUMAN RECOMBINANT SOMATOTROPIN AND ITS MUTANT FORMS BY METAL CHELATE AFFINITY-CHROMATOGRAPHY, Biochemistry, 59(2), 1994, pp. 159-161
Citations number
12
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
2
Year of publication
1994
Pages
159 - 161
Database
ISI
SICI code
0006-2979(1994)59:2<159:AGFPOH>2.0.ZU;2-C
Abstract
A novel scheme for isolation of human recombinant somatotropin and its mutant forms has been developed. The scheme includes ion-exchange and metal chelate affinity chromatography. The chromatographic behavior o f the isolated proteins depends on the amino acid substitutions perfor med.