K. Saegusa et al., Autoantigen-specific CD4(+)CD28(low) T cell subset prevents autoimmune exocrinopathy in murine Sjogren's syndrome, J IMMUNOL, 165(4), 2000, pp. 2251-2257
Organ-specific autoimmune exocrinopathy resembling Sjogren's syndrome (SS)
that spontaneously develops in NFS/sld mutant mice thymectomized 3 day afte
r birth is dependent on Th1-type CD4(+) T cells, We previously reported tha
t a cleavage product of 120-kDa alpha-fodrin may be an important autoantige
n in the pathogenesis of SS in both an animal model and the patients. We de
monstrate that in an animal model of SS with overt exocrinopathy, a unique
CD4(+) T cell subset expressing CD28(low) is dramatically increased in sple
en cells before the disease onset, but that the CD4(+) T cells of diseased
mice were virtually all CD28(high). We found that the spleen cells in these
mice before the disease onset showed a significant increase in autoantigen
-specific T cell proliferation. Analysis of in vitro cytokine production by
spleen cells indicated, before the disease onset, severely impaired produc
tion of IL-2 and IFN-gamma in the animal model, whereas high levels of IL-4
were observed. Expression of cytokine genes, including IL-4, IL-10, and TG
F-beta, was detected in FACS-sorted CD4(+)CD28(low) T cells by RT-PCR analy
sis. Transfer of CD4(+)CD28(low) T cells into the animal model actually pre
vented the development of autoimmune lesions including autoantibody product
ion. These results suggest that a CD4(+)CD28(low) T cell subset that is con
tinuously activated by an organ-specific autoantigen may play a regulatory
role in the development of organ-specific autoimmune disease in an animal m
odel of SS.