An enzymatic method for removal of phenols from their mixtures was investig
ated. Phenols in an aqueous solution were removed after a two-step treatmen
t with co-immobilized laccase and tyrosinase and Polyclar (polyvinylpolypyr
rolidone). A laccase from Pyricularia oryrae and mushroom tyrosinase were c
o-immobilized on Mikroperl in a fixed-bed tubular bioreactor by a rapid and
simple method. The support immobilized 95% of the total laccase units and
35% of the total tyrosinase units. Different mixtures of phenols were passe
d through the column with co-immobilized laccase and tyrosinase, This metho
d removed 42-90% of different phenolic substances by a single passage throu
gh the bioreactor, The second step employed Polyclar for additional removal
of phenolic substances from mixtures. The degree of removal depends on the
nature of the phenols. Complete removal was achieved for alpha-naphthol, 2
,4-dichlorophenol, 4-methoxyphenol, beta-naphthol, 4-chloro-3-methylphenol
and catehin. The operational stability of the immobilized system was 10-90
h depending on the substrate. The biocatalyst was capable of continuous tra
nsformation of different phenols in mixtures.