In this work, different chemical functionalities, both organic and inorgani
c, were inserted in a silica glass based sol-gel derived network to create
specific chemical activities. Modified silica glass networks were prepared
by reacting alkoxysilanes with different chemical functionalities, such as
tetraethoxysilane (TEOS), aminopropyl triethoxysilane (APS) and mercaptopro
pyl triethoxysilane (MPTS), among others. The obtained gels were evaluated
by using infrared spectroscopy, mercury picnometry and electron microscopy.
The chemical activity of the created multifunctional surfaces was evaluate
d by the ability of the incorporated proteins to remain adsorbed onto the d
ifferent gels. Porcine insulin (PI) and bovine serum albumin (BSA) were imp
regnated into modified networks and desorption of those proteins was monito
red. Results showed that gels with multifunctionalities regularly dispersed
can be successfully produced by optimizing some of the processing paramete
rs of the gels, such as pH and concentration of reactants. Results also rev
ealed that the type and concentration of chemical functionalities within th
e gels regulate the ability of incorporated proteins to remain adsorbed on
them, suggesting that chemically patterned surfaces and interfaces can be p
repared which regulate protein-substrate interactions. (C) 2000 Elsevier Sc
ience B.V. All rights reserved.