Recent studies have shown that angiogenesis may be involved in the pathogen
esis of hematopoietic malignancies, apart from its well-characterized role
in the growth and metastasis of solid tumors. In this study, we quantified
the degree of angiogenesis in B cell chronic lymphocytic leukemia (B-CLL) b
y measuring the microvessel density and hotspot density in bone marrow trep
hine biopsy sections with B-CLL involvement (n = 12) and compared it to nor
mal bone marrow sections (n = 11). The B-CLL samples had a mean microvessel
count/high power field (hpf) of 7.64 while the control samples had a mean
microvessel count/hpf of 2.11 (P = 0.0001). The mean hotspot density in the
B-CLL sections (14.83/hotspot) was also significantly higher (P = 0.0008)
than the mean hotspot density in control bone marrow sections (7.09/hotspot
). Both the microvessel density and hotspot density correlated positively w
ith the clinical stage of the B-CLL patients. In a separate cohort of B-CLL
patients, the median urine level of the angiogenic peptide, basic fibrobla
st growth factor (2216.5 pg/g, n = 14), was significantly higher (P = 0.000
1) than the bFGF level in normal controls (1084 pg/g, n = 58). These result
s indicate that angiogenesis may be involved in the pathogenesis of B-CLL.