Homogeneous assays based on deoxyribozyme catalysis

Authors
Stojanovic, MN de Prada, P Landry, DW
Citation
Mn. Stojanovic et al., Homogeneous assays based on deoxyribozyme catalysis, NUCL ACID R, 28(15), 2000, pp. 2915-2918
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEIC ACIDS RESEARCH
ISSN journal
03051048 → ACNP
Volume
28
Issue
15
Year of publication
2000
Pages
2915 - 2918
Database
ISI
SICI code
0305-1048(20000801)28:15<2915:HABODC>2.0.ZU;2-E
Abstract
We report herein the first homogeneous assays based on the ribonuclease act ivity of a deoxyribozyme. The previously reported deoxyribozyme was covalen tly modified with biotin and used to assay biotin-binding interactions thro ugh changes in fluorescence upon substrate turnover. Deoxyribozymes with fl uorescence-based reporting have the potential to serve as general analytica l tools.