GABA immunoreactivity was examined in the retina of the New World monk
ey Cebus apella. Labeled cell bodies were identified as horizontal, bi
polar, interplexiform, amacrine and a population of putative ganglion
cells. To determine whether ganglion cells were immunoreactive to GABA
, double-labeling experiments were performed using Fast Blue as retrog
rade neuronal tracer injected into the superior colliculus. Retinas co
ntaining FB-labeled ganglion cells were subsequently incubated with an
tiserum against GABA. Although retinocollicular ganglion cells were fo
und in three different layers (ganglion cell layer, inner nuclear laye
r and inner plexiform layer), our experiments revealed GABA-positive g
anglion cells only in the outer half of the ganglion cell layer.