A. Hisada et al., Light-induced nuclear translocation of endogenous pea phytochrome A visualized by immunocytochemical procedures, PL CELL, 12(7), 2000, pp. 1063-1078
Although the physiological functions of phytochrome A (PhyA) are now known,
the distribution of endogenous PhyA has not been examined. We have visuali
zed endogenous PhyA apoprotein (PHYA) by immunolabeling cryosections of pea
tissue, using PHYA-deficient mutants as negative controls. By this method,
we examined the distribution of PHYA in different tissues and changes in i
ts intracellular distribution in response to light. In apical hook cells of
etiolated seedlings, PHYA immunolabeling was distributed diffusely in the
cytosol. Exposure to continuous far-red (cFR) light caused a redistribution
of the immunolabeling to the nucleus, first detectable after 1.5 hr and gr
eatest at 4.5 hr. During this time, the amounts of spectrally active phytoc
hrome and PHYA did not decline substantially. Exposure to continuous red (c
R) light or to a brief pulse of red light also resulted in redistribution o
f immunolabeling to the nucleus, but this occurred much more rapidly and wi
th a different pattern of intranuclear distribution than it did in response
to cFR light. Exposures to cR light resulted in loss of immunolabeling, wh
ich was associated with PHYA degradation. These results indicate that the l
ight-induced intracellular location of PHYA is wavelength dependent and imp
ly that this is important for PhyA activity.