ISOLATION, PURIFICATION AND PARTIAL CHARACTERIZATION OF SULFITE OXIDASE FROM MALVA-SYLVESTRIS

Authors
GANAI BA MASOOD A BAIG MA
Citation
Ba. Ganai et al., ISOLATION, PURIFICATION AND PARTIAL CHARACTERIZATION OF SULFITE OXIDASE FROM MALVA-SYLVESTRIS, Phytochemistry, 45(5), 1997, pp. 879-880
Citations number
15
Categorie Soggetti
Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
45
Issue
5
Year of publication
1997
Pages
879 - 880
Database
ISI
SICI code
0031-9422(1997)45:5<879:IPAPCO>2.0.ZU;2-A
Abstract
Sulphite oxidase (EC 1.8.3.1) was isolated from Malva sylvestris leave s and partially characterized. The enzyme was homogeneous with respect to its size and charge, as judged by gel filtration and PAGE. The M-r of the enzyme as estimated by gel filtration was 27 000. The values o f Stokes' radius and frictional ratio were 2.29 nm and 1.16, respectiv ely, and the K-m was 5.33 x 10(-3) M. (C) 1997 Elsevier Science Ltd. A ll rights reserved.