C-METHYL FLAVONOLS FROM THE FUNGUS COLLETOTRICHUM-DEMATIUM F-SP EPILOBII

Two C-6 and C-8 methyl flavonols were isolated from a fungus and ident ified for the first time. The fungus Colletotrichum dematium f.sp. epi lobii is a disease of fireweed, Epilobium angustifolium L. ssp. angust ifolum (family Onagraceae); both disease and plant are indigenous to C anada. C. dematium secretes several metabolites when grown in liquid c ulture. Two novel natural product compounds: 5,4'-dihydfoxy-3,7,8-trim ethoxy-6-C-methylflavone and 5,4'-dihydroxy-3,6,7-trimethoxy-8-C-methy lflavone were isolated from ethyl acetate extracts of liquid culture f iltrates. Structures were confirmed on the basis of UV H-1 NMR, C-13 N MR spectroscopy, as well as mass spectroscopic techniques and represen t the first report of C-methylflavonols produced by fungi. (C) 1997 Pu blished by Elsevier Science Ltd. All rights reserved.