M. Flores et al., Prediction, identification, and artificial selection of DNA rearrangementsin Rhizobium: Toward a natural genomic design, P NAS US, 97(16), 2000, pp. 9138-9143
Citations number
21
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Based on the DNA sequence of the symbiotic plasmid of Rhizobium strain NGR2
34, we predicted potential rearrangements generated by homologous recombina
tion. All predicted rearrangements were identified experimentally by using
a PCR-based methodology. Thus, the predicted and the actual dynamic maps of
the replicon coincide. By using an approach that does not involve the intr
oduction of exogenous genetic elements, derivative populations that are pur
e for specific rearrangements were obtained. We propose that knowledge of t
he DMA sequence of a genome offers the possibility of designing pathways of
sequential rearrangements leading to alternative genomic structures. An ex
perimental strategy to isolate bacterial populations containing the desired
structures is discussed.