In vivo immunological function of mast cells derived from embryonic stem cells: An approach for the rapid analysis of even embryonic lethal mutationsin adult mice in vivo
M. Tsai et al., In vivo immunological function of mast cells derived from embryonic stem cells: An approach for the rapid analysis of even embryonic lethal mutationsin adult mice in vivo, P NAS US, 97(16), 2000, pp. 9186-9190
Citations number
34
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
An important goal of tissue engineering is to achieve reconstitution of spe
cific functionally active cell types by transplantation of differentiated c
ell populations derived from normal or genetically altered embryonic stem c
ells in vitro. We find that mast cells derived in vitro from wild-type or g
enetically manipulated embryonic stem cells can survive and orchestrate imm
unologically specific IgE-dependent reactions after transplantation into ma
st cell-deficient Kit(W)/Kit(W-v) mice. These findings define a unique appr
oach for analyzing the effects of mutations of any genes that are expressed
in mast cells, including embryonic lethal mutations, in vitro or in vivo.