Microsatellite multiplexing in fish

Citation
Bd. Neff et al., Microsatellite multiplexing in fish, T AM FISH S, 129(2), 2000, pp. 584-593
Citations number
38
Categorie Soggetti
Aquatic Sciences
Journal title
TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY
ISSN journal
00028487 → ACNP
Volume
129
Issue
2
Year of publication
2000
Pages
584 - 593
Database
ISI
SICI code
0002-8487(200003)129:2<584:MMIF>2.0.ZU;2-R
Abstract
Microsatellite multiplexing is a powerful technique that can increase the p roductivity of genetic studies in fisheries biology. We review multiplexing methods and present an optimized and detailed protocol For microsatellite multiplexing that is specifically tailored for use with radioisotopes. The protocol can significantly reduce the cost associated with microsatellites and provides high polymerase chain reaction (PCR) fidelity and band resolut ion. Comparing three radioisotopes, we find that end labeling with P-33 pro vides the highest resolution. We also present a quick and inexpensive DNA i solation protocol that is successful with fish larvae. Finally, we End that PCR fidelity depends on the quality of the DNA template, and we therefore review preservation and isolation methods specific to various fish tissue t ypes. Together, these microsatellite multiplexing and DNA isolation protoco ls can significantly reduce the time and expense associated with genetic an alyses in fish.