Microsatellite multiplexing is a powerful technique that can increase the p
roductivity of genetic studies in fisheries biology. We review multiplexing
methods and present an optimized and detailed protocol For microsatellite
multiplexing that is specifically tailored for use with radioisotopes. The
protocol can significantly reduce the cost associated with microsatellites
and provides high polymerase chain reaction (PCR) fidelity and band resolut
ion. Comparing three radioisotopes, we find that end labeling with P-33 pro
vides the highest resolution. We also present a quick and inexpensive DNA i
solation protocol that is successful with fish larvae. Finally, we End that
PCR fidelity depends on the quality of the DNA template, and we therefore
review preservation and isolation methods specific to various fish tissue t
ypes. Together, these microsatellite multiplexing and DNA isolation protoco
ls can significantly reduce the time and expense associated with genetic an
alyses in fish.