Immunologic changes after transfusion of autologous or allogeneic buffy coat-poor versus WBC-reduced blood transfusions in patients undergoing arthroplasty. II. Activation of T cells, macrophages, and cell-mediated lympholysis

BACKGROUND:To estimate the impact of RBC preparations on the status of post operative immune activation, the soluble cytokine receptors of TNF alpha (s TNF-R) and IL-2 (sIL-2R), as well as neopterin and cell-mediated lympholysi s (CML), were measured. STUDY DESIGN AND METHODS: Patients undergoing strictly standardized anesthe siologic management for elective orthopedic surgery were enrolled in a pros pective study. The perioperative course (Days 0, 3, 7, and 10) of sTNF-R, s IL-2R, neopterin, and CML was compared after random assignment to allogenei c buffy coat-reduced (Group 2, n = 8) or WBC-reduced (Group 3, n = 11) RBC transfusion regimen. Recipients of autologous huffy coat-reduced RBC transf usions (Group 1, n = 15) served as controls. Patients receiving intraoperat ively and postoperatively salvaged blood only (n = 10) were separately anal yzed as Group 4. RESULTS: In Group 1, a short-lasting increase in soluble cytokine receptors , a diminished cytolytic response (Day 0 vs. Day 7. sTNF-R, p = 0.0001; sIL -2R, p = 0.0004; CML, p = 0.0238), and an elevation of neopterin (Day 0 vs. Day 3. p = 0.0064) were observed. In contrast, in allogeneically transfuse d patients, sTNF-R (Group 2, p = 0.0469: Group 3, p = 0.0039), sIL-2R (Grou p 3, p = 0.002) and neopterin (Group 3, p = 0.0164) increased further from baseline to Day 10 (Day 0 vs. Day 10), and this increase was accompanied by a diminished cytolytic response (Day 0 vs. Day 10: Group 2, p = 0.05; Grou p 3, p = 0.0076). Patients in Group 4 showed a short-lasting increase in sI L-2R (Day 0 vs. Day 3. p = 0.0078), neopterin (Day 0 vs. Day 3. p = 0.0156) and sTNF-R (Day 0 vs. Day 7. p = 0.0781). CONCLUSION: Allogeneic transfusions seem to prolong the postoperative statu s of immune activation, even when WBC-filtered RBCs are used for the transf usion regimen.