Monoclonal antibodies to Rh D - Development and uses

Monoclonal, anti-D has proved impossible to make in rodent systems. Human m onoclonal anti-D has been produced using EBV transformed peripheral B cells , coupled with fusions to myeloma cell lines. More recently molecular biolo gy techniques have been used to produce monoclonal anti-D. The range of mon oclonal anti-D produced is considered. The selection of monoclonal anti-D f or use as blood grouping reagents for typing donors and recipients is revie wed - all types of D positive should be typed as positive when donors are c onsidered However, D patients should be typed as D negative. Considerations for the development of monoclonal anti-D for prophylactic use are reviewed .