A CD36 synthetic peptide inhibits bleomycin-induced pulmonary inflammationand connective tissue synthesis in the rat

Transforming growth factor (TGF)-beta 1 is an important regulator of inflam mation and fibrosis, TGF-beta 1 is usually secreted as a biologically laten t protein called latent TGF-beta 1 (L-TGF-beta 1). L-TGF-beta 1 has no biol ogic effect unless L-TGF-beta 1 is converted to its active form. Using a we ll-recognized model of lung injury induced by the antineoplastic antibiotic bleomycin (Blm), we demonstrated that 7 d after intratracheal Blm administ ration, total lung TGF-beta was maximally increased. This induction was due to TGF-beta 1 production by alveolar macrophages that, when explanted, gen erated increased quantities of L-TGF-beta 1 complexed with the glycoprotein thrombospondin (TSP)-1. The TSP-1/L-TGF-beta 1 complex was associated with CD36, a receptor for TSP-1. The association of TSP-1/L-TGF-beta 1 to CD36 was critical for plasmin-mediated release of mature TGF-beta 1. In this pap er we show that, compared with administration of Blm by itself, when a synt hetic peptide of CD36 between amino acids 93 and 110 is given concomitantly with Blm to rats, alveolar macrophages generate markedly less active TGF-b eta 1, the rats gain weight more rapidly, and there is less inflammation, c ollagen I and III, and fibronectin synthesis. These findings demonstrate a novel in vivo mechanism of activation of L-TGF-beta 1 in lung injury and th e importance of alveolar macrophage-derived active TGF-beta 1 in the pathog enesis of pulmonary inflammation and fibrosis.