DNA ploidy analysis and cell proliferation in congenital sacrococcygeal teratomas

BACKGROUND. Congenital sacrococcygeal teratoma is the most common germ cell tumor in infants and children. It usually is diagnosed at birth, is benign , and consists of fully differentiated mature tissues. Congenital sacro coc cygeal teratomas (SCTs) also may contain immature tissues, most commonly of neural origin. The proportion of malignant teratomas increases with advanc ing age, but the relation between mature and immature SCTs is not well unde rstood. Thus, it is very important to determine proliferative activity, DNA ploidy, and DNA index to predict biologic behavior of these tumors. METHODS. DNA ploidy and cell proliferation were analyzed by flow cytometry, and the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 were analyzed immunohistochemically on paraffin embedded tissue. RESULTS. All the tumors that were surgically treated within 3 months after birth, including immature teratoma, were diploid. Strongly positive PCNA im munostaining was found in both immature teratomas, and weakly positive PCNA was found in nine cases. Weak positivity for Ki-67 was observed in 2 cases , and moderate positivity was observed in 6 cases including immature terato mas. CONCLUSION. The value of flow cytometry in the prediction of biologic behav ior of congenital SCT should be analyzed further. Our results suggest that Ki-67 and especially PCNA may reflect the proliferative activity of these t umors. (C) 2000 American Cancer Society.