Polymorphism of human platelet alloantigen in Chinese patients with acute myocardial infarction and acute ischemic stroke

Objective To investigate the gene frequencies of 5 major human platelet all oantiqens (HPA 1-5) in Chinese population and to assess if polymorphism of HPA was associated with Chinese acute myocardial infarction (AMI) and acute ischemic stroke (AIS). Methods HPA 1-5 genotyping was performed by PCR using allele specific prime rs and restriction enzyme digestion based on PCR products in 95 AMI cases, 188 AIS cases and 270 normal controls. Gene frequency distribution was test ed by Hardy-Weinberg equilibrium and comparison of HPA gene frequencies bet ween the patient and control groups by chi(2) test. Results The gene frequencies of HPA 1-5 were the followings: HPA1a: 91%; Ib : 9%; HPA 2a: 94%; 2b: 6%; HPA 3a: 83%; 3b: 17%; HPA 4a: 98%; 4b: 2%; HPA5a : 97%; 5b: 3%. We found there were no significant differences in HPA 1-5 ge ne frequencies between AMI patients and normal controls. In AIS patient gro up HPA-2a allele frequency was significant higher than in controls, but thi s allele gene frequency in two groups (0.94 and 0.99) was very close and to o many subjects in these two groups were overlapped. Otherwise no differenc es was found in other 4 HPA systems between cases and controls. Conclusion Polymorphism of HPA were not inherited risky factors and not ass ociated with chinese arterial thrombotic diseases such as AMI and AIS.