Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin

Authors
Zhang, XW Zhou, YH Xu, B
Citation
Xw. Zhang et al., Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin, CHIN MED J, 113(8), 2000, pp. 712-713
Citations number
8
Categorie Soggetti
General & Internal Medicine
Journal title
CHINESE MEDICAL JOURNAL
ISSN journal
03666999 → ACNP
Volume
113
Issue
8
Year of publication
2000
Pages
712 - 713
Database
ISI
SICI code
0366-6999(200008)113:8<712:DOHHHG>2.0.ZU;2-8
Abstract
Objective To study the difterentiation-inducing effect of hydroxycamptothec in (HCPT) on hepatoma Hep G2 cells. Methods 3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyltetrazolinium bromide (MTT ) colorimetric method was used for determining HepG2 cells viability. Alpha fetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits. Results The AFP secretion of HCPT-treated cells was reduced by 44.0% - 49.2 % while the ALE level was increased markedly. Conclusion Hydroxycamptothecin can induce human hepatoma Hep G2 cells to di fferentiation.