Evaluation of the ADVIA (R) Centaur (TM) TSH-3 assay

An analytical evaluation of the thyroid stimulating hormone (TSH-3) assay o n the Sayer ADVIA(R) Centaur(TM) immunoassay system was performed. General analytical requirements (linearity, resistance to typical interferences, ab sence of a carry-over effect) were fulfilled and reproducibility was satisf actory. Inter-assay coefficient of variation (CV) of a human serum pool wit h a concentration of 0.014 mU/l was 22.3%; at concentrations between 0.26 a nd 83 mU/l CV was below 6%. Method comparison study demonstrated close agre ement of TSH results compared to those obtained with the Roche Elecsys(R) 2 010 TSH assay (ADVIA Centaur = 1.08 x Elecsys - 0.18 mU/l; r = 0.987; n = 3 24). Handling and practicability of the ADVIA Centaur system proved to be c onvenient with a very high sample throughput. We conclude that the ADVIA Ce ntaur TSH-3 assay meets requirements for clinical use.