Evaluation of a new simple and rapid enzyme-linked immunosorbent assay kitfor neopterin determination

A new commercially available enzyme-linked immunosorbent assay (ELISA) kit has been evaluated for the measurement of neopterin concentrations in serum , plasma and urine. This competitive ELISA is technically simple, requires only small sample volume and is rapid to perform. The assay procedure consi sts of sequential 1.5 h and 10 min room temperature incubation steps. The E LISA is accurate, sensitive, specific, and precise. Linear regression analy sis of neopterin concentrations measured with the new ELISA and with an est ablished method yielded a highly singificant correlation (r = 0.99). The ne w assay is applicable to ELISA workstations, thus enabling determination of neopterin in large series of samples. The neopterin ELISA kit has been use d in routine laboratory testing of blood donations in a blood bank.