Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2

Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgar is and Vicia faba lectins, The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR, IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the o ther cytokines tested were produced. The IL-8 secreted may induce the extra vasation of activated neutrophils and generate tissue damage. A similar mec hanism may be implicated in the lesions observed after infection by some en teric pathogens, with lectin-like domains on their membrane, Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2 000 Academic Press.