Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microsphere s were used for immobilization of amyloglucosidase and the properties of im mobilized enzyme was studied and compared with those of free enzyme. Sulfon ated groups on the PS/PNaSS microspheres present a very simple, mild, and t ime-saving process for enzyme immobilization. Nonporous microspheres provid e their surface for immobilization of enzyme and prevent the diffusion limi tation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, K-m and V-max, is relatively low compare to other porous supports. Simple and time-saving imm obilization procedure as well as the effects of pH and temperature on immob ilized enzyme also showed that the PS/PNaSS microspheres could be good supp ort. (C) 2000 Elsevier Science Inc. All rights reserved.