Changes in intramitochondrial cardiolipin distribution in apoptosis-resistant HCW-2 cells, derived from the human promyelocytic leukemia HL-60

Using a cytofluorimetric approach, ne studied intramitochondrial cardiolipi n (CL) distribution in HCW-2 cells, an apoptosis-resistant clone of human H L-60 cells. In HL-60, about 50% of total CL is distributed in the outer lea flet of mitochondrial inner membrane, while in HCW-2 a significantly higher amount of CL (about 65%) is in that site. In basal conditions, HSW-2 cells also show a reduced mitochondrial membrane potential even if they are able to proliferate as the parental line. Taking into account the complex funct ions that CL plays in the regulation of mitochondrial activity, it is likel y that HCW-2 could produce. ATP utilizing more glycolytic pathways rather t han mitochondrial respiratory chain. (C) 2000 Federation of European Bioche mical Societies. Published by Elsevier Science B.V. All rights reserved.