Ultrastructural analysis of v-myb oncogene product cooperation with components of avian cell nuclear matrix

The cooperation of the v-Myb oncoprotein with extracted nuclear matrix of a vian haematopoietic cells expressing the v-myb oncogene was studied by mean s of immunoelectron microscopy. The nuclear matrix was extracted by a gentl e method of detergent treatment at moderate ionic strength and visualized e ither in ultrathin LR White sections, in unembedded resin-free sections, an d in addition by the aqueous spreading technique. Using anti-Myb polyclonal antibody we have shown interaction of the v-Myb protein product with extra cted nuclear matrix. This oncoprotein, however, was easily released from th e structure by a detergent as well as by DNAase treatment and ammonium sulp hate extraction. Prefixation of structures before detergent treatment preve nted this extraction, The v-Myb protein marker was distributed in clusters or associated with fibrillar structures in most cases. Single markers decor ating these fibrillar or less dense structures were also detected.