Reduction of oxidative stress and cytokine-induced neutrophil chemoattractant (CINC) expression by red wine polyphenols in zinc deficiency induced intestinal damage of rat

Consumption of red wine has been associated with health promotion and disea se prevention. We have previously found that the intestine of zinc-deficien t (ZD) rats develop oxidative damage associated with inflammation. Here we have used this model to investigate whether red wine polyphenols could prot ect against intestinal injury and, if so, whether this protection was achie ved through antioxidant and anti-inflammatory activity. The intestinal alte rations induced by zinc deficiency such as morphological damage, increased TBA-RS level and CuZn-superoxide dismutase activity, and decreased glutathi one peroxidase activity, did not develop with the administration to ZD rats of a suspension of dealcoholated red wine (RWS). The same treatment induce d in control rats a decrease of TBA-RS level but also of glutathione peroxi dase and catalase activity. Treatment with RWS to ZD rats prevented a marke d mucosal macrophage and neutrophil infiltration. The expression of pro-inf lammatory cytokines, such as tumor necrosis factor a and cytokine-induced n eutrophil chemoattractant (CINC), was induced by zinc deficiency, whereas t hat of the anti-inflammatory interleukin-10 was suppressed. Treatment with RWS reduced CINC expression. These results report a novel activity of red w ine polyphenols in downregulation of intestinal CINC expression, which like ly protects cells against inflammatory processes. (C) 2000 Elsevier Science Inc.