A sequence-ready BAC clone contig of human chromosome 10p15 spanning the loss of heterozygosity region in glioma

Deletion of chromosome 10 is one of the most common chromosomal alterations in glioma. At 10p15, the telomeric region of the short arm of chromosome 1 0, loss of heterozygosity (LOH) has been frequently observed by microsatell ite analysis, suggesting the presence of a tumor suppressor gene. We examin ed LOH in 34 gliomas on chromosome 10, and frequent LOH on 10p was detected on 10p15, in agreement with deletion mapping studies on chromosome 10. We then constructed a bacterial artificial chromosome (BAC) clone contig cover ing the critical region, which spanned the interval between D10S249 and D10 S533 on 10p15. The map contained 68 BAC clones connected by 74 sequenced ta g sites (STSs) and covered approximately 2.7 Mb, with one gap. A total of 7 4 STSs, including 6 microsatellite markers, 29 expressed sequenced tags (ES Ts), and 39 BAC end STSs, were physically arranged. Twenty-eight ESTs were mapped in the interval between D10S249 and D10S559 (approximately 1200 kb), and another EST was mapped in the interval between D10S559 and D10S533 (ap proximately 1300 kb). This sequence-ready BAC clone contig map will be a ba sic resource for high-quality sequencing and positional cloning of the puta tive tumor suppressor gene at 10p15 in glioma. (C) 2000 Academic Press.