The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo

Citation
Mf. Pelletier et al., The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo, GLYCOBIOLOG, 10(8), 2000, pp. 815-827
Citations number
53
Categorie Soggetti
Biochemistry & Biophysics
Journal title
GLYCOBIOLOGY
ISSN journal
09596658 → ACNP
Volume
10
Issue
8
Year of publication
2000
Pages
815 - 827
Database
ISI
SICI code
0959-6658(200008)10:8<815:THSOGI>2.0.ZU;2-Q
Abstract
Glucosidase II is an ER heterodimeric enzyme that cleaves sequentially the two innermost alpha-1,3-linked glucose residues from N-linked oligosacchari des on nascent glycoproteins, This processing allows the binding and releas e of monoglucosylated (Glc(1)Man(9)GlcNAc(2)) glycoproteins with calnexin a nd calreticulin, the lectin-like chaperones of the endoplasmic reticulum, W e have isolated two cDNA isoforms of the human a subunit (alpha 1 and alpha 2) differing by a 66 bp stretch, and a cDNA for the corresponding beta sub unit, The alpha 1 and alpha 2 forms have distinct mobilities on SDS-PAGE an d are expressed in most of the cell lines we have tested, but were absent f rom the glucosidase II-deficient cell line PHA(R) 2,7. Using COS7 cells, th e coexpression of the beta subunit with the catalytic a subunit was found t o be essential for enzymatic activity, solubilization, and/or stability, an d ER retention of the alpha/beta complex, Transfected cell extracts express ing either al or alpha 2 forms with the beta subunit showed similar activit ies, while mutating the nucleophile (D542N) predicted from the glycoside hy drolase Family 31 active site consensus sequence abolished enzymatic activi ty, In order to compare the kinetic parameters of both alpha 1/beta and alp ha 2/beta forms of human glucosidase II the protein was expressed,pith the baculovirus expression system. Expression of the human alpha or beta subuni t alone led to the formation of active human/insect heteroenzymes, demonstr ating functional complementation by the endogenous insect glucosidase II su bunits. The activity of both forms of recombinant human glucosidase II was examined with a p-nitrophenyl alpha-D-glucopyranoside substrate, and a two binding site kinetic model for this substrate was shown. The KM1-2 values a nd apparent Ki1-2 for deoxynojirimycin and castanospermine were determined and found to be identical for both isoforms suggesting they have similar ca talysis and inhibition characteristics, The substrate specificities of both isoforms using the physiological oligosaccharides were assessed and found to be similar.