Complement receptor 2 (CR2) participates in the regulation of B cell respon
ses to antigen. In this study we report that treatment of IM-9 B lymphoblas
toid cells or Raji Burkitt's lymphoma cells with 10% heat-inactivated fetal
bovine serum for 24 hr increased both the CR2 mRNA level and CR2 surface p
rotein expression more than twofold. No change in the CR2 expression level
was observed if cells were cultured in serum-free medium. The CD19 mRNA lev
el decreased after 24 hr independently of the presence of serum. The serum-
stimulated increase in CR2 expression was not due to changes in the prolife
rative capacity of the cells and could not be mimicked by various cytokines
. However, IFN-gamma as well as OKB7, a CR2-specific monoclonal antibody, b
locked the serum-induced increase in CR2 expression at the mRNA level. Our
data show for the first time that factors in serum induce the expression of
the CR2 gene and that signals initiated by IFN-gamma and OKB7 interfere wi
th the serum-induced changes. Because stimuli that alter CR2 expression can
influence the extent of the B cell response to antigen-C3d complexes, seru
m factors may play a role in regulating the responsiveness of B cells.