Use of voltage gradient gel electrophoresis in apoptotic DNA analysis

In this paper the use of voltage gradient gel electrophoresis (VGGE) in the electrophoretic analysis of apoptotic DNAs is described. The peculiarity o f VGGE fractionation in enhancing DNA bands in the gel by reducing their th ickness was used to obtain a rapid, more selective and higher-quality elect rophoretic fractionation of apoptotic DNA with respect to conventional elec trophoresis. The use of VGGE fractionations also allowed a reduced amount o f DNA to be used to detect a characteristic apoptotic DNA ladder pattern, i n a lower agarose gel concentration, with respect to conventional electroph oretic fractionation (C) 2000 Elsevier Science B.V All rights reserved.