Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

A mixed-mode high-performance liquid chromatography (HPLC) method that reso lves the six known non-allelic Variants of chicken erythrocyte histone H1 i s described. Common, but previously unknown, allelic variants of H1 that co migrate in polyacrylamide gel electrophoresis are also resolved. The resolu tion of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses o f post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other p roteins. (C) 2000 Elsevier Science BN. All rights reserved.