Determination of nevirapine, an HIV-1 non-nucleoside reverse transcriptaseinhibitor, in human plasma by reversed-phase high-performance liquid chromatography

A sensitive and rapid high-performance Liquid chromatography method has bee n developed to measure the levels of the HIV-1 non-nucleoside reverse trans criptase inhibitor nevirapine in human plasma. The sample pre-treatment con sists of a protein precipitation with perchloric acid. A Hypersil ODS colum n is used at ambient temperature and a wavelength of 280 nm is used for ult raviolet detection. The mobile phase contains acetonitrile and a 60 mM phos phate buffer pH 4.5 (30:70, v/v). The detection limit of the method is 0.05 mg/l using 150 mu l of plasma. The lower and upper limit of quantitation a re 0.1 mg/l and 10 mg/l, respectively. The average recovery of nevirapine i s 101.8% with a variation of 4.6%. The average inter-assay precision is 2.4 %, the average intra-assay precision 2.9% and the average accuracy 97%. (C) 2000 Elsevier Science BN. All rights reserved.