High-performance liquid chromatographic assay of malagashanine in rat plasma and urine and its pharmacokinetic application

A reversed-phase HPLC method was developed for quantitative analysis of mal agashanine in rat plasma and urine. Malagashanine and internal standard wer e extracted from alkalinized rat plasma. Urine analysis was performed by di rect injection onto the HPLC system. Acetonitrile-aqueous 25 mM sodium acet ate solution at pH 6.25 (45:55, v/v) was used as the mobile phase. The elua te was monitored by using UV detection at 250 nm. The assay was linear with in the concentration range of 10-1000 ng/ml. Both intra- and inter-day accu racy and precision were within acceptable limits. The method was applied to study the pharmacokinetics of malagashanine in rats. (C) 2000 Elsevier Sci ence BN. All rights reserved.