The dynamics of neuronal death: A time-lapse study in the retina

Using time-lapse video microscopy, we have followed the process of neuronal death in an intact region of the mammalian nervous system. We show here th e fast dynamics of nuclear fragmentation, which is over in <1 hr for neuron s undergoing apoptosis in the living rat retina. Nuclear fragmentation is a ccompanied by a progressive raise of intracellular calcium and followed by erratic movement of the apoptotic cells, documenting their loss of adhesion .