High accumulation of plasminogen and tissue plasminogen activator at the flow surface of mural fibrin in the human arterial system

Purpose: We assessed the fibrinolytic activity of the organized mural throm bus lining of aneurysms and prosthetic grafts. Methods: Between May 1995 and April 1998, the full-thickness mural thrombi of aneurysms and the pseudointima lining of vascular grafts were obtained f rom 12 patients, ranging from 55 to 78 years in age, who underwent elective surgery. These included five aortic arch aneurysms, four abdominal aortic aneurysms, and three patent synthetic vascular grafts. The specimens were s ubjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE)/immunoblot and immunohistochemistry for human plasmin/plasminogen, ti ssue plasminogen activator (tPA), and fibrin degradation product (D-dimer). Results: In the SDS-PAGE/immunoblot, 25- and 27-kd bands appeared specifica lly in experimental fibrin plates after limited digestion by plasmin and we re also recognized in the mural thrombi. The presence of bands at 25 and 27 kd, which were most prominent in sections near the flow surface layer, was consistent with the hypothesis that the mural fibrin was digested by the e ndogenous plasmin. Apparent immunoreactivity was found at the flow surface of the masses at a thickness of 10 to 400 mu m, suggesting the presence of a plasminogen and tPA-rich layer, with D-dimer as a consequential product o f fibrinolysis. Conclusion: The hypothesis that fibrin surfaces in the arterial system acqu ire fibrinolytic activity because of digestion by circulating endogenous pl asmin was confirmed; this may contribute to the antithrombogenicity of thes e flow surfaces. (J Vasc Surg 2000;32:374-82.).