Theoretical simulation of oxygen transport to brain by networks of microvessels: Effects of oxygen supply and demand on tissue hypoxia

Objective: Simulations of oxygen delivery by a three-dimensional network of microvessels in rat cerebral cortex were used to examine how the distribut ion of partial pressure of oxygen (Po-2) in tissue depends on blood flow an d oxygen consumption rates. Methods: Network geometry was deduced from previously published scanning el ectron micrographs of corrosion casts. A nonlinear least-squares method, us ing images obtained at three different angles, was used to estimate vessel locations. The network consisted of 50 segments in a region 140 mu m x 150 mu m x 160 mu m. A Green's function method was used to predict the Po-2 dis tribution. Effects of varying perfusion and consumption were examined, rela tive to a control state with consumption 10 cm(3)O(2)/100 g per min and per fusion 160 cm(3)/100 g per min. Results: In the control state, minimum tissue Po-2 was 7 mm Hg. A Krogh-typ e model with the same density of vessels, but with uniform spacing, predict ed a minimum tissue Po-2 of 23 mm Hg. For perfusion below 60% of control, t issue hypoxia (Po-2 <1 mm Hg) was predicted. When perfusion was reduced by 75%, the resulting hypoxia could be eliminated by a 31% reduction in oxygen consumption rate. Conclusions: The simulations suggest that tissue hypoxia resulting from a s evere decrease in brain perfusion, as can occur in stroke, may be avoided b y a moderate decrease in oxygen consumption rate.