Chromosome 20 deletions in myeloid malignancies: reduction of the common deleted region, generation of a PAC/BAC contig and identification of candidate genes
Aj. Bench et al., Chromosome 20 deletions in myeloid malignancies: reduction of the common deleted region, generation of a PAC/BAC contig and identification of candidate genes, ONCOGENE, 19(34), 2000, pp. 3902-3913
Deletion of the long arm of chromosome 20 represents the most common chromo
somal abnormality associated with the myeloproliferative disorders (MPDs) a
nd is also found in other myeloid malignancies including myelodysplastic sy
ndromes (MDS) and acute myeloid leukaemia (AML). Previous studies have iden
tified a common deleted region (CDR) spanning approximately 8 Mb. We have n
ow used G-banding, FISH or microsatellite PCR to analyse 113 patients with
a 20q deletion associated with a myeloid malignancy. Our results define a n
ew MPD CDR of 2.7 Mb, an MDS/AML CDR of 2.6 Mb and a combined 'myeloid' CDR
of 1.7 Mb, We have also constructed the most detailed physical map of this
region to date - a bacterial clone map spanning 5 Mb of the chromosome whi
ch contains 456 bacterial clones and 202 DNA markers. Fifty-one expressed s
equences were localized within this contig of which 37 lie within the MPD C
DR and 20 within the MDS/AML CDR, Of the 16 expressed sequences (sis genes
and 10 unique ESTs) within the 'myeloid' CDR, five were expressed in both n
ormal bone marrow and purified CD34 positive cells. These data identify a s
et of genes which are both positional and expression candidates for the tar
get gene(s) on 20q.