Detection of congenital malaria by polymerase-chain-reaction methodology in Dar es Salaam, Tanzania

The examination of congenital malaria was performed by Giemsa staining and polymerase-chain-reaction (PCR) methodology. We randomly selected 298 neona tes who had been admitted to Muhimbili Medical Center (MMC) at Dar es Salaa m, Tanzania. One baby among all the enrolled neonates was recognized as hav ing a congenital malaria infection, which gave a prevalence of 0.33%. The p resent result was 5-fold the clinically recognized prevalence of congenital infection with malaria in the ward. The PCR method identified two cases, o ne of which was negative as determined by the Giemsa-staining method. There fore, the PCR method was useful for the detection of scant amounts of malar ial parasites in numerous blood samples. The screening of malaria by a sens itive PCR method contributes to reduce the mortality of asymptotic neonates in particular.