Specific protocols need to be developed that take into consideration the re
quirements of silver foxes. This study was designed to investigate the repr
oduction of 26 pairs of silver foxes (Vulpes vulpes). Reproduction paramete
rs (breeding season, age at puberty, fecundity and fertility) of the foxes
were examined under experimental conditions, and new techniques to optimize
fox breeding were assessed. Staining of the vaginal smears with the double
Harris-Schorr stain allowed for the precise detection of estrus and proved
to be more reliable than simply measuring vaginal resistance. Ultrasonogra
phy was demonstrated to be useful for pregnancy diagnosis and prediction of
parturition. Results demonstrate that the methods reported in this paper a
re easily applicable to similar studies carried out on small groups of anim
als within the framework of fox animal experimentation. (C) 2000 by Elsevie
r Science Inc.