The mating-type region of Schizosaccharomyces pombe h(-S) 972: sequencing and analysis of 69 kb including the expressed mat1 locus

The sequence has been determined of 68 897 bp of genomic DNA including the expressed mat1 mating-type locus from Schizosaccharomyces pombe h(-S) strai n 972. The DNA sequence, located on the long arm of fission yeast chromosom e II and contained in two cosmid clones, was analysed to reveal one autonom ously replicating sequence, two retrotransposon long terminal repeats (LTRs ), one tRNA(Gly) gene and 33 open reading frames (ORFs), of which 15 contai n introns. Nine of these ORFs code for previously described genes (tut1, rp l10, rps21, nif1, sui1 (psu1), matMi, matMc, let1 and rpa4), one of which ( trt1) contains 15 introns, the highest number yet recorded in a gene of S. pombe. Of the remaining 24 ORFs, sequence similarity suggests that the func tion of 13 of the encoded proteins may be predicted and these include four mitochondrial proteins, two transport proteins, two signalling molecules, a component of serine palmitolytransferase, a homologue of 3-methyladenine D NA glycosylase, a multifunctional alcohol dehydrogenase, a killer toxin sen sitivity factor and an acetyl transferase. Six deduced sequences appear to be related to proteins of unknown function in Saccharomyces cerevisiae or S , pombe and the remaining five are hypothetical proteins. This sequence has been submitted to the EMBL database under the following entries: SPBC23G7 (Accession No. AL035065), SPBC18E5 (AL035077) and SPBC29A3 (part) (AL022299 ). Copyright (C) 2000 John Whey & Sons, Ltd.