At. Woestenburg et al., Morphometric analysis of renal vessels - Advantage of the multisector areaapproach to cross-sections, ANAL QUAN C, 22(4), 2000, pp. 285-290
Citations number
7
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
OBJECTIVE: To determine the most accurate morphometric approach to overcomi
ng the obstacles of limited number, angle of section and irregular contours
of vascular structures in analyzing vascular sections of renal biopsies.
STUDY DESIGN: The luminal area of 451 cortical arterioles in 65 Masson-tric
home-stained renal sections uns assessed with a computer-assisted imaging s
ystem connected to a Leica DMR microscope (Mikroskopic und Systeme GmbH, We
tzlar Germany). The luminal area measured by the imaging system was used as
the gold standard, against which three mathematical sector approaches and
one classical approach were evaluated. The accuracy of these approaches was
evaluated by means of the relative deviation from the measured value ann o
f the degree of overestimation or underestimation. Intraobserver and intero
bserver variability were determined for the most accurate mathematical appr
oach.
RESULTS: As compared to measured luminal area, the sector elliptical approa
ch yielded the lowest relative deviation (13.4 +/- 12.5%), without signific
ant overestimation ol underestimation (-0.6 +/- 18.3%). The intraobserver a
nd interobserver correlation coefficients for this method were 82.3% and 86
.5%, respectively.
CONCLUSION: The sector elliptical approach is the most accurate mathematica
l approach to vascular sections in renal biopsies.