Survival and growth of Litopenaeus vannamei (Boone) larvae fed a single dose of live algae and artificial diets with supplements

The present work evaluates the effectiveness of partial or total replacemen t of microalgae with artificial diets [CAR Frippak. experimental Dry Immune Selco (DIS, with vitamins C, E, HUFA and immunostimulants)] administered t ogether with a single dose of live algae (SDLA) for the protozoeal stages o f the Pacific white shrimp Litopenaeus vannamei (Boone) (expt 1). In expt 2 , Artemia enriched with DIS were fed to mysis l-postlarvae 1 to assess diet performances. The results indicate that highest survival and growth is obt ained when larvae at the N6-PZ1 moult are administered DIS (2 mg L-1 day(-1 )) + CAR (2 mg L-1 day(-1)) and SDW (20 cells mu L-1 mixed algae, Chaetocer os muelleri and Isochrysis galbana, 70%:30% respectively) as a first feed, and DIS + CAR (2 mg L-1 day(-1) each) thereafter until PZ3 (survival 91.2%) , as opposed to live microalgae control (81.9%). A significantly better tot al length was achieved by larvae fed the live microalgae control (2.51 mm) or the above artificial diet regime (2.44 mm) than in all other treatments. Larvae administered this artificial diet treatment were significantly heav ier (0.039 mg larvae(-1)) than those fed the algal control (0.023 mg larvae (-1)) or any other treatment. This may result from the combined effect of t he artificial diet together with vitamins C, E and HUFA in the booster DIS. These results demonstrate for the first time the impact that a single firs t feed at the N6-PZ1 moult may have on later development, as all larval gro ups received the same diet from the PZ1 to PZ3 stages. The results from exp t 2 indicate that the administration of Artemia enriched with DIS during my sis 1-post-larvae 1 for L. vannamei larvae results in significant increase in survival (88.6%), total length (5.24 mm) and weight (0.155 mg larvae(-1) ) in comparison with other enrichments, such as DIS 5V1 (without vitamins, but with HUFA) or non-enrichment.