P. Saldarelli et al., Immunodetection and subcellular localization of the proteins encoded by ORF 3 of grapevine viruses A and B, ARCH VIROL, 145(8), 2000, pp. 1535-1542
Polyclonal antisera were raised to Escherichia coli-expressed ORF3 products
(putative move ment proteins) of Grapevine virus A (GVA) and Grapevine vir
us B (GVB) (genus Vitivirus), and used for their immunodetection in infecte
d plants. Western blot analysis of subfractionated cellular compartments sh
owed that the distribution of both proteins was comparable to that of plant
virus movement proteins, as they were transiently present in a crude membr
ane fraction and accumulated in a cell wall-enriched fraction. The GVA ORF3
-encoded protein, but not the comparable GVB protein, was also present in l
arge amount in a cytoplasmic soluble fraction. Intracellular immunogold lab
elling localized these proteins in the cell wall and plasmodesmata of infec
ted cells and, especially for GVA, in association with cytoplasmic virus ag
gregates.