Sphenoclea zeylanica (gooseweed), a major weed of paddy rice in Southeast A
sia, is one of the targets in a biological weed control research program in
the Philippines. A fungal pathogen, Alternaria alternata f. sp. sphenoclea
e, is being evaluated as a biological control agent for this weed. The feas
ibility of solid substrate fermentation for the mass production of A. alter
nata f. sp. sphenocleae has been examined. Conidia production and virulence
of A. alternata f. sp. sphenocleae were affected by temperature, light, an
d incubation period. Abundant conidia were produced under continuous light
on seeds of sorghum, hard red spring wheat, and barley at 28 degrees C. The
greatest number of conidia was produced on sorghum seed followed by barley
and oats seeds at 28 degrees C exposed to near-ultraviolet (NUV). More con
idia were produced at 28 degrees C under NUV light on sorghum, barley, oats
, and hard red spring wheat seeds, cornmeal, and polished rice grains than
on the other substrates. Less conidia were produced on these substrates und
er light. At 28 degrees C, large numbers of virulent conidia were produced
on sorghum seeds after 4 weeks of incubation under either constant light or
dark. A mix of equal quantities of sorghum seeds and water (w/v) maximized
conidial production. Conidia produced on sorghum seeds had a shelf life of
at least 12 months when stored in production flasks under room conditions
(24+/-2 degrees C). The use of sorghum seeds as a solid substrate for produ
ction of A. alternata f. sp. sphenocleae could be a feasible method to prod
uce conidia in a village co-operative or cottage industry type scenario in
Southeast Asia.