A high-throughput screening utilizing intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Authors
Matsumoto, C Hamasaki, K Mihara, H Ueno, A
Citation
C. Matsumoto et al., A high-throughput screening utilizing intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically, BIOORG MED, 10(16), 2000, pp. 1857-1861
Citations number
14
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
10
Issue
16
Year of publication
2000
Pages
1857 - 1861
Database
ISI
SICI code
0960-894X(20000821)10:16<1857:AHSUIF>2.0.ZU;2-V
Abstract
A 16-residue peptide, including the Tat(49-57) sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respective ly. This double dye-labeled peptide was prepared as a tracer for high-throu ghput screening utilizing intramolecular fluorescence resonance energy tran sfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reser ved.